Predicting the impact of hydraulic retention time and biodegradability on the performance of sludge acidogenesis using an artificial neural network.

This study aimed to predict volatile fatty acids (VFAs) production from SDBS-pretreated waste-activated sludge (WAS). A lab-scale continuous experiment was conducted at varying hydraulic retention times (HRTs) of 7 d to 1 d. The highest VFA yield considering the WAS biodegradability was 86.8 % based on COD at an HRT of 2 d, where the hydrolysis and acidogenesis showed the highest microbial activities. According to 16S rRNA gene analysis, the most abundant bacterial class and genus at an HRT of 2 d were Synergistia and Aminobacterium, respectively. Training regression (R) for TVFA and VFA yield was 0.9321 and 0.9679, respectively, verifying the efficiency of the ANN model in learning the relationship between the input variables and reactor performance. The prediction outcome was verified with R2 values of 0.9416 and 0.8906 for TVFA and VFA yield, respectively. These results would be useful in designing, operating, and controlling WAS treatment processes.

Recent updates in biohydrogen production strategies and life-cycle assessment for sustainable future.

Biohydrogen (bio-H2) is regarded as a clean, non-toxic, energy carrier and has enormous potential for transforming fossil fuel-based economy. The development of a continuous high-rate H2 production with low-cost economics following an environmentally friendly approach should be admired for technology demonstration. Thus, the current review discusses the biotechnological and thermochemical pathways for H2 production. Thermochemical conversion involves pyrolysis and gasification routes, while biotechnological involves light-dependent processes (e.g., direct and indirect photolysis, photo/ dark fermentation strategies). Moreover, environmentally friendly technologies can be created while utilizing renewable energy sources including lignocellulosic, wastewater, sludge, microalgae, and others, which are still being developed. Lifecycle assessment (LCA) evaluates and integrates the economic, environmental, and social performance of H2 production from biomass, microalgae, and biochar. Moreover, system boundaries evaluation, i.e., global warming potential, acidification, eutrophication, and sensitivity analysis could lead in development of sustainable bioenergy transition with high economic and environmental benefits.

Critical challenges and technological breakthroughs in food waste hydrolysis and detoxification for fuels and chemicals production.

Organic waste has increased as the global population and economy have grown exponentially. Food waste (FW) is posing a severe environmental issue because of mismanaged disposal techniques, which frequently result in the squandering of carbohydrate-rich feedstocks. In an advanced valorization strategy, organic material in FW can be used as a viable carbon source for microbial digestion and hence for the generation of value-added compounds. In comparison to traditional feedstocks, a modest pretreatment of the FW stream utilizing chemical, biochemical, or thermochemical techniques can extract bulk of sugars for microbial digestion. Pretreatment produces a large number of toxins and inhibitors that affect bacterial fuel and chemical conversion processes. Thus, the current review scrutinizes the FW structure, pretreatment methods (e.g., physical, chemical, physicochemical, and biological), and various strategies for detoxification before microbial fermentation into renewable chemical production. Technological and commercial challenges and future perspectives for FW integrated biorefineries have also been outlined.

Recent advances in black liquor valorization.

Lignocellulosic biomass is projected as a prospective renewable alternative to petroleum for the production of fuel and chemicals. Pretreatment is necessary to disrupt the lignocellulosic structure for extraction of cellulose. Biomass after pretreatment is segregated into cellulose rich solid fraction and black liquor (lignin and hemicelluloses) as a liquid stream. The plant polysaccharide-based industry primarily utilizes the cellulosic fraction as raw material, and carbon rich black liquor discarded as waste or burnt for energy recovery. This review highlights the recent advancements in the biological and chemical valorization of black liquor into fuels and chemicals. The recent research attempted for bioconversion of black liquor into Bioplastic, Biohydrogen, Biogas, and chemicals has been discussed. In addition, the efforts to replace the conventional energy recovery method with the advanced chemical process along with their modifications have been reviewed that will decide the sustainability of the lignocellulosic biomass-based industry.

Design and applications of photobioreactors- a review.

There has been increasing attention in recent years on the use of photobioreactors for various biotechnological applications, especially for the cultivation of microalgae. Photobioreactors-based production of photosynthetic microorganisms furnish several advantages as minimising toxicity and providing improved conditions. However, the designing and scaling-up of photobioreactors (PBRs) remain a challenge. Due to huge capital investment and operating cost, there is a deficiency of suitable PBRs for development of photosynthetic microorganisms on large-scale. It is, therefore, highly desirable to understand the current state-of-the-art PBRs, their advantages and limitations so as to classify different PBRs as per their most suited applications. This review provides a holistic overview of the discreet features of diverse PBR designs and their purpose in microalgae growth and biohydrogen production and also summarizes the recent development in use of hybrid PBRs to increase their working efficiency and overall economics of their operation for the production of value-added products.

Harnessing fruit waste for poly-3-hydroxybutyrate production: A review.

Poly-3-hydroxybutyrate is a biopolymer which has shown tremendous potential for replacing conventional petroleum-based plastics for plummeting the plastic pollution problem. However, the production cost of PHB is high which makes it less attractive for commercial use. To tackle this challenge, various researchers suggest the search for low-cost substrates and energy efficient technologies for PHB production. In this regard, the waste generated from fruit processing industries or fruit wastes could be pre-processed and fermented for effectively generating PHB. Therefore, the aim of this review was to focus on the methods of fruit waste pre-processing and the effect of fermentation variables on PHB production using fruit waste as a substrate. The relevant research findings on the use of different microorganisms, PHB production conditions and fruit waste-based substrates are also covered. Analysis of various studies revealed that pineapple and mixed fruit waste are effective for PHB production.